Beneficial using of EDTA to reduce cadmium toxicity and to improve the physiological and biochemical profiles of catfish (Clarias gariepinus)

Ramadan, Ashraf; Shalaby, Adel M.; El Gammal, Mostafa A.; El Aganif, El-Sadiq M El Aganif M; Ebrahim, Mohamed S.

doi:10.21608/eja.2011.32050

Beneficial using of EDTA to reduce cadmium toxicity and to improve the physiological and biochemical profiles of catfish (Clarias gariepinus)

Authors

¹ Fish Genetics Department, Central Laboratory for Aquaculture Research, Abbassa, Abo- Hammad, Sharkia, Egypt.

² Hatchary and Fish Physiology Department, Central Laboratory for Aquaculture Research, Abbassa, Abo- Hammad, Sharkia, Egypt.

³ -Fish limnology Departement, Central Laboratory for Aquaculture Research, Abbassa, Abo- Hammad, Sharkia, Egypt.

⁴ Aquaculture Department – Faculty of Agriculture , Trapoiils University, libya.

⁵ Fish Ecology Department Central Laboratory for Aquaculture Research, Abbassa, Abo- Hammad, Sharkia, Egypt.

10.21608/eja.2011.32050

Abstract

EDTA form metal-EDTA complexes which have its effectiveness in mobilizing of the contaminant metal ions, especially cadmium. This work was carried out to investigate the effect of the ion –exchanging (chelating) agent EDTA on cadmium (Cd) toxicity and its impact on haematological and biochemical changes in catfish (Clarias gariepinu)). The fish (160-180g ) were exposed to 12 ppm Cd alone or with 0.1, 0.2 and 0.3 g EDTA/L for 3, 10 and 45 days. Cd exposure reduced significantly (P<0.05) erythrocyte count (RBCs), haemoglobin content (Hb), haematocrit value (Hct), mean cell volume (MCV) mean cell haemoglobin (MCH) and mean cell haemoglobin concentration (MCHC). These parameters were improved when EDTA was applied with Cd. The values of RBCs , Hb, Hct, MCV, MCH and MCHC were increased significantly to be as in the control fish group. There was significant decreases in plasma total protein (TP) in fish exposed to Cd alone. The levels of plasma glucose, total lipids (LP), aspartate aminotranseferase (AST), alanine aminotransferase (ALT) were increased significantly in fish exposed to Cd alone. Addition of EDTA to Cd contaminated medium enhanced biochemical parameters in fish and the enzyme activities returned to be as the control fish group. Addition of EDTA to Cd contaminated medium considerably reduced metal absorption and its accumulation in fish tissues, and reduced metals in water. Fish exposed to Cd alone accumulates 2.93, 4.33 and 7.2 mg Cd/g dry weight in body fish for 3, 10 and 45 days, respectively. Cd was reduced significantly to 0.13, 0.17 and 0.22 mg Cd/g dry weight in fishes exposed to 0.1, 0.2 and 0.3g EDTA/L for 3,10 and 45 days, respectively these values were similar to those of control group.