Blue Resources for Aquaculture, Environment and Society Development
Egyptian Journal for Aquaculture
2090-7877
2636-3984
8
1
2018
03
01
Efficacy of agglutination test in diagnosis of motile Aeromonas septicemia in Nile tilapia (Oreochromis niloticus)
1
12
EN
Mahmoud
Mohamed
Eladawy
Researcher, Fish health and management Department , Central Laboratory for Aquaculture Research , Egypt
eladawy3m83@gmail.com
Ahmed
Ali
Fish desease Department, CentralLaboratory for Aquaculture Research, ARC, Egypt
Ahmed
Abdel Wahab
Fish desease department, Central laboratory for Aquaculture Research, ARC, Egypt
ahmedgaber869@gmail.com
10.21608/eja.2018.44524
Bacterial diseases are the most common diseases among cultured fish. Aeromonas hydrophila considered the most important freshwater bacterial fish pathogen. Early diagnosis considered the cornerstone in controlling the disease. Biochemical method is laborious, expensive and time consuming whereas agglutination test is simple, rapid, accurate inexpensive and the test can be performed in field conditions. In the present study, agglutination test was established for diagnosis A. hydrophila infection in Oreochromis niloticus. A. hydrophila was isolated from naturally infected fish then distracted to subunits somatic and flagellar antigens. The prepared antigen was reacted with positive antisera of A. hydrophila which produced distinct tiny clumps. The sensitivity and specificity of slide agglutination test against collected sera were 90%, 77.5% respectively in case of somatic antigen and 92.5%, 72.5% in case of flagellar antigen. It could be concluded that agglutination test can be used as screen test for diagnosis of A.hydrophila infection in fish farms and the method can be diagramed on other bacteria.
aeromonas,agglutination,antigen and Oreochromis niloticus
https://eja.journals.ekb.eg/article_44524.html
https://eja.journals.ekb.eg/article_44524_8970cad9b7186d5fee1afdce8f1f1181.pdf
Blue Resources for Aquaculture, Environment and Society Development
Egyptian Journal for Aquaculture
2090-7877
2636-3984
8
1
2018
03
01
Investigation of Acanthocephalan parasites in some marine fishes as a bio-indicator for heavy metals pollution
13
30
EN
Maather
El-lamie
Faculty of Vetrenary Medicen, Suez Canal University
maather76@yahoo.com
Heba
I.
Abdel-Mawla
Department of Fish Diseases and Management Animal Health Res. Institute (Ismailia Branch).
10.21608/eja.2018.68355
A total of 120 marine fish as (60 <em>Siganus revulatus</em> and 60 <em>Mulloides flavolineatus</em>) were collected from Suez Canal area at Suez Governorate to investigatethe presence of acanthocephalan parasites and its relation with some heavy metals. Residues of lead (Pb), zinc (Zn), copper (Cu), cadmium, (Cd) and iron (Fe) were detected in water, acanthocephalan parasites and in the organs of infested and non-infested fishes (livers, gills, and musculature) of the examined fishes using atomic absorption spectrophotometer. The clinical picture of naturally infested fishes showed no pathognomonic signs except abdominal distension or emaciation in some fishes. Postmortem lesions were in pale liver and internal organs with presence of acanthocephalan in the opened viscera or perforated intestine. Some cases showed congestion in the liver with petechial hemorrhage. The total prevalence of infested fishes with acanthocephalans was 30%. The isolated Acanthocephala were identified as <em>Neohydinorhynchus macrospinosus </em>from<em>S. revulatus </em>36.66 % and <em>Echinorhynchus sp., </em><em>Serrasentis sagittifer</em><em> from M. flavolineatus </em>23.33%. The morphological descriptions of each isolated parasites were described.
The present study indicated that the average of heavy metal residues in organs of the infested fishes were lower than its residues in organs of the non-infested fishes. Also, the acanthocephalan parasites are able to accumulate heavy metals in their tissues higher than fish tissues and
could be considered as a bio-indicator for heavy metals pollution besides being a competitor to fish organs for heavy metals accumulation.
The obtained results of heavy metals in the edible parts of the fish demonstrated at the safe levels for human consumption and its concentrations in the musculature are generally accepted by the worldwide enactment limits.
marine fish,Siganus revulatus,Mulloides flavolineatus,acanthocephalan,bio-indicator,Heavy metals,Lead,cadmium,iron,pollution
https://eja.journals.ekb.eg/article_68355.html
https://eja.journals.ekb.eg/article_68355_93c5a3cbf471e17a69e27c19287a5a80.pdf
Blue Resources for Aquaculture, Environment and Society Development
Egyptian Journal for Aquaculture
2090-7877
2636-3984
8
1
2018
03
01
Influence different levels of Oregano extract ( Origanum vulgare ) as Food Additives on growth performance, chemical composition and blood components for Oreochromis niloticus fingerlings under semi-intensive culture conditions
31
52
EN
Ahmed
Fath El-Bab
Animal production, Faculty of Agriculture, Damietta University, Egypt.
ah_farouk74@yahoo.com
M.
M.
El-Moghazy
Animal production, Faculty of Agriculture, Damietta University, Egypt
M.
T.
Shehab El-Din
Fish Health, Central laboratory for Aquaculture Research,
(Sakha Unit), Egypt.
Eslam
A.
Mohameed
Animal production, Faculty of Agriculture, Damietta University, Egypt.
10.21608/eja.2018.68356
<span class="fontstyle0">The aim of this study is to investigate the effect of oregano (</span><span class="fontstyle2">Origanum vulgare</span><span class="fontstyle0">) by different levels (control,<br />0.05, 0.10, 0.15 and 0.20% </span><span class="fontstyle2">Origanum vulgare</span><span class="fontstyle0">) in </span><span class="fontstyle2">Oreochromis niloticus </span><span class="fontstyle0">diets on Growth performance<br />parameters, histology and chemical analysis of sex reversed males of Nile tilapia </span><span class="fontstyle2">O. niloticus</span><span class="fontstyle0">. Ten concrete<br />ponds 3x7 x lm (2l m</span><span class="fontstyle0">3 </span><span class="fontstyle0">for each – semi intensive culture) were used in this study. The ten ponds represented 5<br />treatments (with 2 replicates for each treatment), and each pond was stocked with lo fish / m</span><span class="fontstyle0">3 </span><span class="fontstyle0">(2l0 fish for each pond)<br />with an initial weights average ranged between 4.90 and 5.02 ± 0.67gm for five treatments, respectively.<br />Fingerlings were fed at a rate of 5% of the total fish mass until the end of experimental period (l6 weeks). All diets<br />contained 30% crude protein and about 360 Kcal/100 g digestible energy. fish fed these diets twice daily for six<br />days a week. The duration of the experiment was l6 weeks. The results obtained from this experiments showed<br />that: -<br />The highest rate of final body weight (FBW), daily weight gain (DWG), final body length (FBL), specific growth rate (SGR) and protein efficiency ratio (PER), were recorded for the third treatment (0.10% </span><span class="fontstyle2">Origanum</span> <span class="fontstyle0">vulgare</span><span class="fontstyle1">). while, the first treatment (control) was recorded the lowest growth parameters (FBW, DWG, FBL, SGR and PER).<br />The chemical composition for the whole fish, the second treatment was recorded the highest rate of protein% and the third treatment recorded the highest rate of fat% and the first treatment recorded the highest rate of ash%. As for the edible part of the fish, the second treatment was recorded the highest rate of protein and ash % and the third treatment recorded the highest rate of fat%. With regard for the Blood components, the fifth treatment had the highest rate of hemoglobin and creatinine and the third treatment was recorded the highest rate of red blood cells and the first treatment recorded the highest rate of liver functions and cholesterol. The second treatment recorded the highest rate of total protein and albumin.<br />Based on the results obtained in this study, it can be concluded that the use of 0.10% oregano extract was best for growth, liver function and chemical composition under semi-intensive culture.</span>
oregano,Origanum vulgare,Intensive culture,Growth performance,O. niloticus
https://eja.journals.ekb.eg/article_68356.html
https://eja.journals.ekb.eg/article_68356_e183c517b3069762841a244b737c4a52.pdf