Blue Resources for Aquaculture, Environment and Society Development
Egyptian Journal for Aquaculture
2090-7877
2636-3984
9
1
2019
01
01
Effect of different dietary protein-energy ratio on growth, feed utilization, body composition and haematological indices of European sea bass, Dicentrarchus labrax fingerlings
1
23
EN
Ashraf
Goda
National Institute of Oceanography and Fisheries (NIOF), Alexandria, Egypt
Tarek
Srour
Fish and Animal Production Dep., Faculty of Agriculture (Saba Basha), Alexandria University, Egypt
Abdallah
Mansour
Fish and Animal Production Dep., Faculty of Agriculture (Saba Basha), Alexandria University, Egypt
Mohamed
Baromh
National Institute of Oceanography and Fisheries (NIOF), Alexandria, Egypt
Hani
Nazmi
National Institute of Oceanography and Fisheries (NIOF), Alexandria, Egypt
Ahmed
Baromh
National Institute of Oceanography and Fisheries (NIOF), Alexandria, Egypt
10.21608/eja.2019.31513
This study was conducted to investigate the effect three dietary protein energy ratio on growth performance, feed utilization and haematological indices of European sea bass <em>Dicentrarchus labrax</em> fingerlings culture in net enclosures (hapa, 30 m<sup>-3 </sup>each) for 60 days. The experimental treatments were assigned in triplicate. Three test diets were formulated to contain three different protein levels (450, 500, and 550 g kg<sup>-1</sup> diet) and three lipid levels (174, 160 and 150 g kg<sup>-1</sup> diet) to provided three different dietary protein: energy ratio (P: E ratio: 20 , 23 and 25 mg CP kJ<sup>-1</sup> GE g<sup>-1</sup>, P:E<sub>21</sub>, P:E<sub>23 </sub>and P:E<sub>25</sub>, respectively). A total of 180 European sea bass, <em>Dicentrarchus labrax</em> with an average initial body weight of 13.0 ± 0.5 g fish<sup>-1</sup> were randomly distributed into nine net enclosures measuring (3 × 8 ×1.25m each) at a stocking density of 20 fish per net enclosure. Over the 60-days feeding period, growth, feed utilization efficiency and survival (%) of <em>D. labrax</em> fingerlings was improved significantly (P>0.05) with increasing dietary protein energy ratio up to P:E<sub>23</sub>compared to P:E<sub>25</sub> diets. The same trend was observed for the best feed conversion ratio (FCR). No statistical difference (P ≥ 0.05) was observed for the influence of dietary protein energy ratio on whole body proximate analysis of fish except for protein which recorded the highest values for fish fed P:E<sub>23 </sub>diet. Mean red blood cell counts (RBCs), mean white blood cell counts (WBCs), hematocrit (Hct), hemoglobin (Hb), total plasma protein, total plasma globulin, plasma sodium chloride and glucose of <em>D. labrax</em> fingerlings significantly (P ≥ 0.05) increased for fish fed the diets P:E<sub>25 </sub>compared to other experimental treatments. An opposite trend was observed for plasma cortisol, albumin, plasma aspartate aminotransferase (AST) and alanine aminotransferase (ALT) values. The obtained findings revealed that, the diet containing 500 g kg<sup>-1</sup> protein and 160 g kg<sup>-1</sup> lipid with dietary P: E ratio of 23 mg CP kJ GE g<sup>-1</sup> is recommended to stimulate growth performance, nutrients utilization efficiency and haematological indices of <em>D. labrax</em> fingerlings
Dicentrarchus labrax,protein,energy,Lipid,haematological,Growth
https://eja.journals.ekb.eg/article_31513.html
https://eja.journals.ekb.eg/article_31513_9d7bfbf64a670254589523f3d5459338.pdf
Blue Resources for Aquaculture, Environment and Society Development
Egyptian Journal for Aquaculture
2090-7877
2636-3984
9
1
2019
03
01
Use of high protein distiller’s dried grains (HPDDG) with enzyme phytase as a cost effective ingredient in the diet of fingerlings European sea bass, Dicentrarchus labrax
25
48
EN
Ashraf
Goda
National Institute of Oceanography and Fisheries (NIOF), Alexandria, Egypt.
Sherine
Ahmed
Ragab
National Institute of Oceanography and Fisheries (NIOF), Alexandria, Egypt.
sherine_a78@yahoo.com
Abdallah
Mansour
Fish and Animal Production Department, Faculty of Agriculture (Saba Basha), Alexandria University, Egypt.
Mohamed
Baromh
National Institute of Oceanography and Fisheries (NIOF), Alexandria, Egypt.
Hani
Nazmi
National Institute of Oceanography and Fisheries (NIOF), Alexandria, Egypt.
Eglal
Omar
Fish and Animal Production Department, Faculty of Agriculture (Saba Basha), Alexandria University, Egypt.
Tarek
Srour
Fish and Animal Production Department, Faculty of Agriculture (Saba Basha), Alexandria University, Egypt.
10.21608/eja.2019.33051
The present study was conducted to evaluate the effect of various dietary levels of high protein distiller’s dried grains (HPDDG) supplemented with enzyme phytase on growth performance, feed utilization and haematological indices of European sea bass Dicentrarchus labrax fingerlings. A total of 150 D. labrax fingerlings with an average body weight of 7.5 ± 0.5g were divided in the five experimental treatments (in triplicates each). The experiment was conducted for 56 days. Fish were fed to apparent the satiation six days a week. Five isonitrogenous 45% CP experimental diets were formulated. The control (C) diet had no high protein distiller’s dried grains (HPDDG) and phytase enzyme added. Diet 2 (C+) was formulated as control diet and supplemented with phytase at a level of 0.5 g/kg. Diets 3-5 each contained HPDDG at levels of 20, 30, and 40 %, respectively with soybean meal and supplemented each with phytase at a level of 0.5 g/kg. Over the 56 days feeding period, all fish fed HPDDG-supplemented with phytase diets resulted in higher growth than the control diet, suggesting that the using of HPDDG-supplemented with phytase enhanced the growth performance of sea bass fingerlings. The same trend was recorded for the best FCR and PER. The hematology and serum biochemistry (hemoglobin (Hb), red blood cells (RBCs) count, white blood cells (WBCs) and hematocrit (Hct %) and immune parameters (total protein, albumin, globulin, cholestorl, lysozyme activity and total antioxidant capacity) of D. labrax fingerlings significantly (P ≥ 0.05) increased with increasing dietary HPDDG with phytase. Result indicates that HPDDG with phytase is a good alternative protein source for compromising growth performance and physiological parameters of European sea bass, Dicentrarchus labrax Fingerlings
Dicentrarchus labrax,high protein distillers dried grains,phytase,physiological parameters,Growth
https://eja.journals.ekb.eg/article_33051.html
https://eja.journals.ekb.eg/article_33051_b4bfb8b96a116119841c7383684c0473.pdf
Blue Resources for Aquaculture, Environment and Society Development
Egyptian Journal for Aquaculture
2090-7877
2636-3984
9
1
2019
03
01
Effect of rice straw on chemical properties and heavy metals residues in the bottom sediment of fish pond
49
60
EN
Enas
Mohamed
Mansour
limnology department, Central Laboratory For Aquaculture Research, Abbassa, Abou-Hammad, Sharkia, Egypt.
enasgalal82@gmail.com
Naglaa
Shalaby
limnology department, Central Laboratory For Aquaculture Research, Abbassa, Abou-Hammad, Sharkia, Egypt.
Niema
Aly
Fish Biology and Ecology department, Central Laboratory For Aquaculture Research, Abbassa, Abou-Hammad, Sharkia, Egypt.
neema_ali62@yahoo.com
10.21608/eja.2019.33104
This study was conducted to determine the effect of rice straw on some chemical properties and heavy metals accumulation of fish pond bottom sediment in earthen fish ponds. The present work had been conducted in the world fish center during the period from May to December 2017 in six earthen fish ponds having equal areas (1000 m2) and 1 m water depth All ponds cultured with mono six Nile tilapia fry in the rate of 3 fingerling/ m2. All ponds were fertilized with dry chicken manure in the rate of 15 kg/pond/week, and divided into two treatments, each of 3 replicates; the first one (T1), act as control, while the second (T2) received 45 kg dry rice straw/pond. Samples were collected at the beggining and at the end of the experiment; from both treatments as well as from the source of water. The study showed that the sediment pH lied in the alkaline side and negatively correlated with organic matter contents. Concentrations of organic matter and organic carbon were significantly higher in T2 than T1, indicating that rice straw increased total carbon in the bottom sediment. Total nitrogen and total phosphorus (TP) concentrations were varied significantly among treatments. The highest value had been recorded in T2. The different investigated parameters were significantly increased at the end of experiment compared to the beggining, expect for C/N and N/P ratios which decreased at the end of the experiment. Concerning the investigated heavy metals; their concentrations had the, following order: Fe > Zn > Cu > Pb > Cd and tended to be higher in the source water then in T1, while the lowest concentrations had been observed in T2. It's observed that the metals concentrations were significantly decreased at the end of experiment compared to the beginning expect for Cd which increased at the end of experiment. The results of this investigation indicated that rice straw improved sediment characteristics, increased nitrogen availability and decreased the investigated heavy metals residues.
chemical properties,Heavy metals,bottom sediment,rice straw
https://eja.journals.ekb.eg/article_33104.html
https://eja.journals.ekb.eg/article_33104_25b640a4945ea02c19522c4ba7698cbb.pdf
Blue Resources for Aquaculture, Environment and Society Development
Egyptian Journal for Aquaculture
2090-7877
2636-3984
9
1
2019
03
01
Effects of commercial and fish isolated gut Bacillus strains supplementation on the performance of Oreochromis niloticus challenged with Aeromonas hydrophila.
61
75
EN
Mai
E.
Naser
Department of Pharmacology, Faculty of Veterinary Medicine, Zagazig University, 44511 Zagazig, Egypt.
Sabry
M.
Abd El-Motaal
Department of Pharmacology, Faculty of Veterinary Medicine, Zagazig University, 44511 Zagazig, Egypt.
Sawsan
M.
El-Sheikh
Department of Pharmacology, Faculty of Veterinary Medicine, Zagazig University, 44511 Zagazig, Egypt.
Shahira
H.
Mahmoud
Animal Health Research institute, 44511 Zagazig, Egypt.
10.21608/eja.2019.33105
Apparently healthy <em>Oreochromis niloticus</em> (n = 225) were used to investigate the effect of commercial probiotic (<em>B. subtilis</em>) or fish gut isolated <em>Bacillus</em> strains (<em>B. amyloliquefaciens</em>; 7 H N and <em>B. cereus</em>; 29 H N) on growth performance, immune response, some serum biochemical parameters and disease resistance against <em>Aeromonas hydrophila</em> infections. They were randomly assigned to four treatments with three replicates per treatment (45 fish/ treatment; 15 fish/replicate except control group has six replicates). The experimental fish were fed with diets supplemented with 0 (control), 1 x 10<sup>6</sup> CFU g<sup>-1</sup> fed commercial <em>B. subtilis</em> or gut isolated <em>B. amyloliquefaciens</em> (7 H N) and <em>B. cereus</em> (29 H N) for 2 months then bacterial challenge were performed at the end of the experiment. Fish fed gut isolated<em> B. cereus</em> (29 H N) had the highest marked (<em>p<0.05</em>) influence on the productive performance parameters among treated groups. Furthermore, the highest levels of nitric oxide, Immunoglobulin (IgM) and lysozyme were found in treated groups. Infected fish fed on gut isolated <em>B. cereus</em> had a positive effect on ALT, SOD activity while creatinine and urea were marked reduction compared with other dietary treatments. It could be inferred that gut isolated <em>Bacillus</em> has been suggested as growth promoter, immune-stimulant in <em>O. niloticus</em> and increase protection against <em>A. hydrophila</em> infection. Among the two isolated <em>Bacillus</em> species, dietary supplementation with the <em>B. cereus</em> had the highest performance in <em>O. niloticus</em> compared with the commercial <em>B. subtilis</em>.
Bacillus,O. niloticus,Growth,immunity,biochemistry,Aeromonas hydrophila
https://eja.journals.ekb.eg/article_33105.html
https://eja.journals.ekb.eg/article_33105_557b47d60acd64fddd531050d3f8b461.pdf
Blue Resources for Aquaculture, Environment and Society Development
Egyptian Journal for Aquaculture
2090-7877
2636-3984
9
1
2019
01
01
Biochemical changes of Moringa seeds extracts on Nile Tilapia breeding fish
77
95
EN
Emad
M
Gad
Chemistry Department, faculty of science, Suez Canal University.
Ahmed
Abd El-Rahman
Fish physiology Department, central Laboratory for Aquaculture Research, Abbassa, Abo-Hammad, Sharkia, ARC, Egypt.
Adel
M.
Shalaby
Fish physiology Department, central Laboratory for Aquaculture Research, Abbassa, Abo-Hammad, Sharkia, ARC, Egypt.
Shimaa
M
Metwally
Fish physiology Department, central Laboratory for Aquaculture Research, Abbassa, Abo-Hammad, Sharkia, ARC, Egypt.
shimaametwally2015@gmail.com
10.21608/eja.2019.48769
The present study was conducted to evaluate the effect of <em>Moringa oleifera</em> seeds extracts in two different solvents ethanol and petroleum ether on the reproductive performance and physiological state of Nile Tilapia. Fish were stocked in 15 concrete ponds, grouped into 5 treatments in triplicates for each treatment. The first treatment T1 (control without any moringa extract), the second T2 and the third T3 (containing respectively 0.5 g and 1 g of moringa seed ethanol extract (MSEE)/kg diet), the fourth T4 and the fifth T5 (containing respectively 0.5 g and 1 g of moringa seed petroleum ether extract (MSPEE)/kg diet). The experiment extended to 70 days. The results showed that the highest numbers of fry production and ovaries weights were obtained with T4 and T5. The best treatment for blood hematological parameters is T4 and the highest level of testosterone and esteradiol hormones were observed in all moringa treatments. Thus it was concluded that 0.5 g of Moringa seed petroleum ether extract/kg was useful for the improvement of the reproductive performance and physiological blood characteristics of brooding stock Nile tilapia fish.
Moringa oleifera,seeds,extracts,biochemical,Nile tilapia,and Breeding
https://eja.journals.ekb.eg/article_48769.html
https://eja.journals.ekb.eg/article_48769_0c8dd28696cc4bdcb76eba055af9c30e.pdf