ORIGINAL_ARTICLE
Studies on yersiniosis in cultured Mugil seheli for the first time in Suez Governorate
A total of 360 cultured Mugil seheli had clinical signs were collected from private farms in Suez Governorate, Egypt. M. seheli was subjected to clinical, post -mortem and bacteriological examinations for detection of Yersinia ruckeri. Yersinia ruckeri was identified by biochemical reactions and polymerase chain reaction (PCR). One isolate of Y. ruckeri. The results revealed that the presence of Yersinia ruckeri infection was 68.1%. The highest prevalence of Y. ruckeri infection in cultured M. seheli was during autumn season (80%) followed by spring (72.2%), summer (70%) and then winter (50%). PCR amplification of DNA from Y. ruckeri isolates using 16s rRNA (YER8/10) specific primers for Y. ruckeri resulted in PCR products size of 575 bp. All Y. ruckeri isolates were found to be sensitive to Ciprofloxacin, Tobramycin and Trimethoprim. While resisted Erythromycin, Amoxycillin and Novobiocine.
https://eja.journals.ekb.eg/article_31474_dc8ac105e0577fee33b9b1e8673b3a86.pdf
2017-06-01
1
14
10.21608/eja.2017.31474
Ismail
Eissa
1
Fish Diseases and Management Dept. Faculty of Vet. Med., Suez Canal University
AUTHOR
Ahmed
El-Ashram
elashram_aquahealth@yahoo.com
2
Fish Health and Diseases Dept. Faculty of Fish Resources, Suez University, Egypt.
LEAD_AUTHOR
Yasmine
El-Barbary
3
Fish Diseases and Management Dept. Faculty of Vet. Med., Suez Canal University
AUTHOR
ORIGINAL_ARTICLE
Effect of basil oil (Ocimum basilicum) on nonspecific immune response of Nile-tilapia (Oreochromis niloticus)
Bacterial fish diseases are considered as major constraint in aquaculture production. The use of natural phytochemicals is promising in aquaculture because they are safe for the environment and human health, biocompatible and biodegradable. The present study was carried out to investigate the effect of Ocimum basilicum oil on the non-specific immune response of Nile-tilapia (Oreochromis niloticus) and resistance against Aeromonas hydrophila infection. A hundred and eighty Nile-tilapia (Oreochromis niloticus) (20±2 g/fish) were randomly distributed and divided into four equal groups in well prepared glass aquaria supplied with continuous areation. The 1st group was assigned as non-treated control group. The 2nd, 3rd and 4th groups were fed on treated ration with basil oil at concentration of 0.25, 0.5 and 1% of basil oil /kg diet for 42 days, respectively. The obtained results showed that the orally administrated basil oil has been shown to enhance the non-specific immune response in the treated fish (hematocrit levels, respiratory burst activity, serum lysozyme and serum bactericidal activity) all over the experiment period in compared with the control group. Also, significant decrease in total bacterial count of fish muscles in compared with control group. Mortality rates post challenge infection, were significantly lesser in treated groups in compared with control group. It was observed that the mortalities among the challenged fish are dose related. These findings are supportive of the potential of basil oil use as ecofriendly alternative measures of disease prevention for sustainable aquaculture.
https://eja.journals.ekb.eg/article_31475_592f59761788dde1acfcf4a30cc4c62e.pdf
2017-06-01
15
31
10.21608/eja.2017.31475
basil oil
Oreochromis niloticus
non-specific immune response
Aeromonas hydrophila
Ahmed
El-Ashram
elashram_aquahealth@yahoo.com
1
Fish Health and Diseases Dept. Faculty of Fish Resources, Suez University, Egypt.
AUTHOR
Abeer
Afifi
abeeraquaculture@gmail.com
2
Fish Diseases Dept., Central Lab. for Aquaculture Research, Abbassa, Egypt.
LEAD_AUTHOR
Saleh
Sakr
3
Fish Diseases Dept., Central Lab. for Aquaculture Research, Abbassa, Egypt.
AUTHOR