@article { author = {Eladawy, Mahmoud and Ali, Ahmed and Abdel Wahab, Ahmed}, title = {Efficacy of agglutination test in diagnosis of motile Aeromonas septicemia in Nile tilapia (Oreochromis niloticus)}, journal = {Egyptian Journal for Aquaculture}, volume = {8}, number = {1}, pages = {1-12}, year = {2018}, publisher = {Blue Resources for Aquaculture, Environment and Society Development}, issn = {2090-7877}, eissn = {2636-3984}, doi = {10.21608/eja.2018.44524}, abstract = {Bacterial diseases are the most common diseases among cultured fish. Aeromonas hydrophila considered the most important freshwater bacterial fish pathogen. Early diagnosis considered the cornerstone in controlling the disease. Biochemical method is laborious, expensive and time consuming whereas agglutination test is simple, rapid, accurate inexpensive and the test can be performed in field conditions. In the present study, agglutination test was established for diagnosis A. hydrophila infection in Oreochromis niloticus. A. hydrophila was isolated from naturally infected fish then distracted to subunits somatic and flagellar antigens. The prepared antigen was reacted with positive antisera of A. hydrophila which produced distinct tiny clumps. The sensitivity and specificity of slide agglutination test against collected sera were 90%, 77.5% respectively in case of somatic antigen and 92.5%, 72.5% in case of flagellar antigen. It could be concluded that agglutination test can be used as screen test for diagnosis of A.hydrophila infection in fish farms and the method can be diagramed on other bacteria.}, keywords = {aeromonas,agglutination,antigen and Oreochromis niloticus}, url = {https://eja.journals.ekb.eg/article_44524.html}, eprint = {https://eja.journals.ekb.eg/article_44524_8970cad9b7186d5fee1afdce8f1f1181.pdf} } @article { author = {El-lamie, Maather and Abdel-Mawla, Heba}, title = {Investigation of Acanthocephalan parasites in some marine fishes as a bio-indicator for heavy metals pollution}, journal = {Egyptian Journal for Aquaculture}, volume = {8}, number = {1}, pages = {13-30}, year = {2018}, publisher = {Blue Resources for Aquaculture, Environment and Society Development}, issn = {2090-7877}, eissn = {2636-3984}, doi = {10.21608/eja.2018.68355}, abstract = {A total of 120 marine fish as (60 Siganus revulatus and 60 Mulloides flavolineatus) were collected from Suez Canal area at Suez Governorate to investigatethe presence of acanthocephalan parasites and its relation with some heavy metals. Residues of lead (Pb), zinc (Zn), copper (Cu), cadmium, (Cd) and iron (Fe) were detected in water, acanthocephalan parasites and in the organs of infested and non-infested fishes (livers, gills, and musculature) of the examined fishes using atomic absorption spectrophotometer. The clinical picture of naturally infested fishes showed no pathognomonic signs except abdominal distension or emaciation in some fishes. Postmortem lesions were in pale liver and internal organs with presence of acanthocephalan in the opened viscera or perforated intestine. Some cases showed congestion in the liver with petechial hemorrhage. The total prevalence of infested fishes with acanthocephalans was 30%. The isolated Acanthocephala were identified as Neohydinorhynchus macrospinosus fromS. revulatus 36.66 % and Echinorhynchus sp., Serrasentis sagittifer from M. flavolineatus 23.33%. The morphological descriptions of each isolated parasites were described. The present study indicated that the average of heavy metal residues in organs of the infested fishes were lower than its residues in organs of the non-infested fishes. Also, the acanthocephalan parasites are able to accumulate heavy metals in their tissues higher than fish tissues and  could be considered as a bio-indicator for heavy metals pollution besides being a competitor to fish organs for heavy metals accumulation. The obtained results of heavy metals in the edible parts of the fish demonstrated at the safe levels for human consumption and its concentrations in the musculature are generally accepted by the worldwide enactment limits.}, keywords = {marine fish,Siganus revulatus,Mulloides flavolineatus,acanthocephalan,bio-indicator,Heavy metals,Lead,cadmium,iron,pollution}, url = {https://eja.journals.ekb.eg/article_68355.html}, eprint = {https://eja.journals.ekb.eg/article_68355_93c5a3cbf471e17a69e27c19287a5a80.pdf} } @article { author = {Fath El-Bab, Ahmed and El-Moghazy, M. and Shehab El-Din, M. and Mohameed, Eslam}, title = {Influence different levels of Oregano extract ( Origanum vulgare ) as Food Additives on growth performance, chemical composition and blood components for Oreochromis niloticus fingerlings under semi-intensive culture conditions}, journal = {Egyptian Journal for Aquaculture}, volume = {8}, number = {1}, pages = {31-52}, year = {2018}, publisher = {Blue Resources for Aquaculture, Environment and Society Development}, issn = {2090-7877}, eissn = {2636-3984}, doi = {10.21608/eja.2018.68356}, abstract = {The aim of this study is to investigate the effect of oregano (Origanum vulgare) by different levels (control,0.05, 0.10, 0.15 and 0.20% Origanum vulgare) in Oreochromis niloticus diets on Growth performanceparameters, histology and chemical analysis of sex reversed males of Nile tilapia O. niloticus. Ten concreteponds 3x7 x lm (2l m3 for each – semi intensive culture) were used in this study. The ten ponds represented 5treatments (with 2 replicates for each treatment), and each pond was stocked with lo fish / m3 (2l0 fish for each pond)with an initial weights average ranged between 4.90 and 5.02 ± 0.67gm for five treatments, respectively.Fingerlings were fed at a rate of 5% of the total fish mass until the end of experimental period (l6 weeks). All dietscontained 30% crude protein and about 360 Kcal/100 g digestible energy. fish fed these diets twice daily for sixdays a week. The duration of the experiment was l6 weeks. The results obtained from this experiments showedthat: -The highest rate of final body weight (FBW), daily weight gain (DWG), final body length (FBL), specific growth rate (SGR) and protein efficiency ratio (PER), were recorded for the third treatment (0.10% Origanum vulgare). while, the first treatment (control) was recorded the lowest growth parameters (FBW, DWG, FBL, SGR and PER).The chemical composition for the whole fish, the second treatment was recorded the highest rate of protein% and the third treatment recorded the highest rate of fat% and the first treatment recorded the highest rate of ash%. As for the edible part of the fish, the second treatment was recorded the highest rate of protein and ash % and the third treatment recorded the highest rate of fat%. With regard for the Blood components, the fifth treatment had the highest rate of hemoglobin and creatinine and the third treatment was recorded the highest rate of red blood cells and the first treatment recorded the highest rate of liver functions and cholesterol. The second treatment recorded the highest rate of total protein and albumin.Based on the results obtained in this study, it can be concluded that the use of 0.10% oregano extract was best for growth, liver function and chemical composition under semi-intensive culture.}, keywords = {oregano,Origanum vulgare,Intensive culture,Growth performance,O. niloticus}, url = {https://eja.journals.ekb.eg/article_68356.html}, eprint = {https://eja.journals.ekb.eg/article_68356_e183c517b3069762841a244b737c4a52.pdf} }